Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, functional overexpression of His6-tagged enzyme in Escherichia coli strain BL21 codon Plus, subcloning in Escherichia coli strain XL 10 GOLD, TcDELTA9DES-expressing Escherichia coli cells exhibit high desaturase activity associated with increased levels of monounsaturated fatty acids. The fatty acid composition of Escherichia coli shows that the recombinant desaturase can use 16:0 as substrate | Trypanosoma cruzi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ketoconazole | - |
Trypanosoma cruzi |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | membrane-bound | Trypanosoma cruzi | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ | Trypanosoma cruzi | - |
1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ | Trypanosoma cruzi Tulahuen | - |
1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | G8FL60 | - |
- |
Trypanosoma cruzi Tulahuen | G8FL60 | - |
- |
Purification (Comment) | Organism |
---|---|
native enzyme from epimastigotes partially by ultracentrifugation, recombinant His6-tagged enzyme from Escherichia coli strain BL21 codon Plus cell-free extract by nickel affinity chromatography | Trypanosoma cruzi |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epimastigote | - |
Trypanosoma cruzi | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ | - |
Trypanosoma cruzi | 1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ | - |
Trypanosoma cruzi Tulahuen | 1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, about, recombinant enzyme, SDS-PAGE | Trypanosoma cruzi |
Synonyms | Comment | Organism |
---|---|---|
9des | - |
Trypanosoma cruzi |
DELTA9 desaturase | - |
Trypanosoma cruzi |
TcDELTA9DES | - |
Trypanosoma cruzi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
in vivo assay at | Trypanosoma cruzi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Ferredoxin | - |
Trypanosoma cruzi |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Trypanosoma cruzi | about, isoelectric focusing | - |
9 |
General Information | Comment | Organism |
---|---|---|
metabolism | desaturases, key enzymes in the metabolism of fatty acids, regulate the physical and biochemical properties of membranes. They adjust the composition of saturated and unsaturated fatty acids in response to changes in the environmental. This DELTA9-desaturation reaction normally requires molecular oxygen and reducing equivalents derived from NAD(P)H through an electron system consisting of either NADPH, cytochrome b5 and cytochrome b5-reductase in animals and fungi or ferredoxin-NADP+ oxidoreductase and ferredoxin in bacteria and plants | Trypanosoma cruzi |
physiological function | the enzyme plays a critical role in the metabolism of the parasite | Trypanosoma cruzi |